Marcador Mezclado Smg de proteínas is a ready-to-use three-color protein standard with 10 pre-stained proteins covering a wide range of molecular weights from 6.5 to 270 kDa. Proteins are covalently coupled with a blue chromophore except for three reference bands (two orange bands at 30 kDa and 270 kDa and one green band at 52 kDa) when separated on SDS-PAGE (Tris-glycine buffer). The MIXED Protein Ladder is designed for observing protein separation during SDS- polyacrylamide gel electrophoresis and verifying Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of proteins. The ladder is supplied in the gel loading buffer. Available in a pack of 5 with 500ml in each tube.

CAT No.: SMGBM-1MZC

Size Range: 6.5-270 kDa

Volume: 500ml each tube

Application of Solmeglas SMG Mixed Protein Markers:

• SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Features of Solmeglas SMG Mixed Protein Markers:

• Solutions are prepared to be ready to load
• Visible during run time
• Bands have precisely defined sizes
• 25 and 72 kDa have brighter intensity for aiding in identification

Content: Storage Buffer

• 5 mM Tris-H3PO4 (pH 7.5 at 25 °C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3, 33% glycerol

Storage:

• Store at 25°C for up to 2 weeks, 4°C for up to 3 months and -20°C for long term storage

Quality Control:

• Under suggested conditions, MIXED Protein Ladder resolves 10 major bands in 4-12% Bis-Tris Gel (MES buffer) and after Western blotting to the nitrocellulose membrane.