Vector de expresión-pRCR Plasmid

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1.- Mohedano, M.L., García-Cayuela, Pérez-RamosA., Rogier A. Gaiser, A.R., Requena, T., López, P. (2015). Construction and validation of a mCherry protein vector for promoter analysis in Lactobacillus acidophilus. J. Ind. Microbiol. Biotechnol. DOI: 10.1007/s10295-014-1567-4.

2.- Russo, P., Iturria, I., Mohedano, M.L., Caggianiello, G., Rainieri, S., Fiocco, D., Pardo, M.A., López, P., Spano, G (2015). Zebrafish gut colonization by mCherry-labelled acid bacteria. Appl Microbiol Biotechnol 99(8): 3479-3490. DOI: 10.1007/s00253-014-6351-x.

3.- Nácher-Vázquez, M., Ruiz-Masó, J. A., Mohedano, M. L., del Solar, G., Aznar, R. and López, P. 2017. Dextransucrase expression is concomitant with that of replication and maintenance functions of the pMN1 plasmid in Lactobacillus sakei MN1. Front. Microbiol. 8:2281.

4.- Pérez-Ramos, A., Werning, M.L., Prieto, A., Russo, P., Spano, G. Mohedano, M.L., López, P*. 2017. Characterization of the sorbitol utilization cluster of the probiotic Pediococcus parvulus 2.6: genetic, functional and complementation studies in heterologous hosts. Front. Microbiol., 8: 2393.



pRCR plasmid in Escherichia coli, Lactococcus lactisLactobacillus species and Streptococcus pneumoniae.

Vector information

Vector type: plasmid

Vector size (kb): 4.1

Markers: catR

Reporter gene: mrfp

Replicons: pSH71

Possible hosts:  E. coli, L. lactis, Lactobacillus species and S. pneumoniae.

Suggested hosts: E. coli DH5α, L. lactis MG1363, S. pneumoniae R6, Lactobacillus spp. type strains for gene manipulation and testing.