Vector de expresión – pLS1ROM



1.- Ruiz-Masó JA, López-Aguilar C, Nieto C, Sanz M, Burón P, Espinosa M, del Solar, G. 2012. Construction of a plasmid vector based on the pMV158 replicon for cloning and inducible gene expression in Streptococcus pneumoniae. Plasmid 67(1): 53-59. doi:10.1016/j.plasmid.2011.09.001.

2.- Chan WT, Yeo CC,  Sadowy E, and Espinosa M. 2014. Functional validation of putative toxin-antitoxin genes from the Gram-positive pathogen Streptococcus pneumoniae: phd-doc is the fourth bona-fide operon. Front. Microbiol. 5:677. doi:10.3389/fmicb.2014.00677.

3.- Eutsey RA, Powell E, Dordel J, Salter SJ, Clark TA, Korlach J, Ehrlich GD, Hiller NL. 2015. Genetic stabilization of the drug-resistant PMEN1 pneumococcus lineage by its distinctive DpnIII restriction-modification system. mBio 6(3): e00173-15. doi:10.1128/mBio.00173-15.



pLS1ROM plasmid, which has been tested to be functional in Streptococcus pneumoniae, Lactococcus lactisStaphylococcus aureus and Listeria monocytogenes.

Vector information

Vector type: plasmid

Vector size (kb): 6.8

GenBank: JN381945.1

Markers: ermR,

Promoter: PM

Replicon: pMV158

Possible hosts: Firmicutes

Suggested hosts: S. pneumoniae R6, L. lactis MG1363, S. aureus RN4220, L. monocytogenes EGD