Mastermix Red contains red dye for visualization and pipetting accuracy. A very popular alternative to the regular and colourless mastermix with red dye that can be removed by spin column-based nucleic acid technique. The red dye of this mastermix will not interfere with the PCR process and product. It is a ready-to-use solution that also contains Taq DNA polymerase, MgCl ₂ and dNTPs to allow amplification of nucleic acid templates by polymerase chain reaction (PCR). This is recommended for any kind of PCR application like standard PCR, high-yield PCR, fast PCR and etc. Storage at 4 ° C avoids the necessity of thawing the mix before assembling the PCR.No detectable reduction of PCR performance or enzyme activity observed after storage of Mastermix Red for 12 months at 4 ° C. Repeated freeze-thaw cycles will not reduce performance or activity. Available in 100rxns (2x 1.25ml).

CAT No .: SMGBM1-RMM

Size: 100 rxns

Applications of Mastermix Red from Solmeglas:

• Standard PCR
• High-yield PCR
• Fast PCR
• Diagnostic PCR
• High throughput applications
• DNA Amplification with low copy number genes
• TA Cloning

Features of Solmeglas Mastermix Red:

• Inactive at room temperature
• Convenient
• Produces consistent results
• Direct gel loading
• Reduced contamination risks
• High purity
• Leaves adenine bases overhang at 3 ‘bases
• Processes up to 5kb
• No need for a separate loading dye
• Can be loaded into agarose or SDS gels
• Good for visualisation and pipetting accuracy

Storage: Store at -20 ° C or 4 ° C upon receiving

Shelf Life: 2 years after production date

Quality Control:

• Evaluated by DNA polymerisation activity assay to measure the percentage of Taq DNA polymerase in inhibited and uninhibited control.
• The functional assay is also performed.
• Components are tested for the absence of DNA, RNAse and exonuclease activities.
• Recombinant Taq DNA polymerase is tested for the absence of exonuclease and single- and double-stranded exonuclease activities.
• The enzyme is more than 90% homogenous as determined by SDS polyacrylamide gel electrophoresis.

Guidelines and Recommendations:

• Every appropriate precaution has to be taken into account to avoid cross-contamination.
• Amplification reaction is suggested to be carried out in a DNA-free environment.
• Usage of aerosol-resistant barrier tips is recommended.
• Special care should be taken to prevent contamination with primers or DNA template between individual reactions. PCR products should be analyzed in a separate area from the reaction assembly area.
• In a standard 50 μl reaction, 25 μl of Mastermix Red is used and the remaining 25 μl for the addition of DNA templates.
• If it is required to adjust the Mg ²⁺ concentration, the volume has to be included in the solution in order to achieve the final reaction volume of 50 μl.

Protocol:

The following protocol is suggested as a guideline when using Mastermix Red. We recommend placing the reactions on ice from pre-chilled components. This protocol is for a reaction size of approximately 50 μl. The reaction size may be adjusted as desired.

Note: For multiple reactions with common components, prepare a master mix of the components common to all reactions to reduce pipetting errors.

1. Set up reaction tubes/plates on ice.
2. Add the following components to the reaction tube/vessel.

• 25 μl 2X PCR SuperMix
• Primers (200 nM final concentration per first is recommended) *
• Template DNA solution *

* Total volume of primer and template solution should be 25 μl.

3. Mix contents and cover with mineral or silicone oil, if necessary.
4. Close the caps and load the reaction tubes in a thermal cycler.
5. Run the cycling program.