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ZetaView® BASIC – NTA Nanoparticle Tracking – Video Microscope PMX-120

For the analysis of size, concentration, fluorescence, electrophoretic mobility and sub-populations of individual nanoparticles. Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or viruses-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the Particle Metrix ZetaView®.

 

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Key Features of ZetaView® BASIC:

  • Sensitive CMOS camera that makes it efficient to see small nanoparticles in fluorescence
  • Option of combining Zeta Potential NTA (Z-NTA) and Fluorescence NTA (F-NTA) functions in one
  • ZetaView® BASIC is the platform on which ZetaView® TWIN and ZetaView® QUATT are built. Therefore, the series offers upgradability making budgetary decisions easier.
  • No other nanoparticle analysis method offers more analysis parameters per sample. At high throughput, in scattering as well as in fluorescent mode.

 

Typical Application of ZetaView® BASIC:

Nanoparticle Tracking Analysis (NTA) with ZetaView® BASIC, TWIN and QUATT works by capturing the Brownian motion of each particle in a video.

 

Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined.

 

Furthermore, the charge state of the particle surface (zeta potential) can be measured via the movement of the particles in an applied electric field.

 

Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles as well as particle concentration are documented at each recording and can be used to distinguish sub-populations.

 

All these analyzes are carried out quickly and statistically reliably as required in scattered light or fluorescence mode.

 

Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labelled with corresponding intercalating dyes.

 

Depending on the type of sample and the measuring mode, the measuring range is between 15nm and 5μm.

 

Benefits as with ZetaView® TWIN PMX-120 and ZetaView® QUATT PMX-420:

  • Very short measuring times. Over 1000 particles can be analyzed in as little as 60 seconds
  • Ready-to-use calibration standard
  • Recovery of samples
  • Can measure 11 distinct positions offering a more representative analysis than single-point measurements
  • Results are outlier-free
  • Low bleach technology. Described as a sensitive CMOS camera combined with high filtering capability and short illumination time, this provides ZetaView® with a high F-NTA sensitivity. F-NTA in turn provides specific information about subpopulations in the sample
  • Fully automatic focusing of the optics keeps video quality constant, removes subjective user input bias and saves the user time in preparing the instrument for use
  • Quartz cell channel and easy to clean
  • Standard operating practice methodology
  • Applicable in the good manufacturing practice range
  • Installation Qualification (IQ) / Operational Qualification (OQ) / Performance Qualification (PQ)
  • No high-cost consumables – just syringes and reference standards for scattering and fluorescence measurements

 

Try out the ZetaView® NTA-Simulator

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