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For the analysis of size, concentration, fluorescence, electrophoretic mobility and sub-populations of individual nanoparticles. Biological nanoparticles such as extracellular vesicles (EVs), exosomes, viruses, or viruses-like particles are a rapidly growing area of research in the life sciences and nanomedical field. The rapid in vitro measurement of multiple physical parameters such as size, concentration, surface charge, and phenotype characteristics is the special feature of the Particle Metrix ZetaView®.
Nanoparticle Tracking Analysis (NTA) with ZetaView® BASIC, TWIN and QUATT works by capturing the Brownian motion of each particle in a video.
Based on the different diffusion movements of large and small particles in the surrounding liquid, the hydrodynamic diameter of the particles is determined.
Furthermore, the charge state of the particle surface (zeta potential) can be measured via the movement of the particles in an applied electric field.
Pattern parameters, such as intensity fluctuations, surface geometry and shape of the particles as well as particle concentration are documented at each recording and can be used to distinguish sub-populations.
All these analyzes are carried out quickly and statistically reliably as required in scattered light or fluorescence mode.
Antibody-conjugated EVs can be differentiated from membrane-enveloped vesicles labelled with corresponding intercalating dyes.
Depending on the type of sample and the measuring mode, the measuring range is between 15nm and 5μm.